Phosphorus Pentoxide as a Drying Agent for Bacterial Culture Extracts Analyzed by Gas-Liquid Chromatography

Analysis of acetoin and diacetyl in bacterial culture supernatants by gas-liquid chromatography.

The acetoin and diacetyl contents of culture supernatants of Voges-Proskauer-positive "viridans" streptotocci, Klebsiella pneumoniae and Staphylococcus aureus, were determined by a gas liquid chromatographic procedure, in which supernatants were extracted with diethyl ether and diacetyl was measured on columns of 10% (wt/wt) polyethylene glycol 400 (PEG 400) at 73 C. Acetoin was converted to di...

Rapid differentiation of bacterial meningitides by direct gas-liquid chromatography.

Rapid identification of Haemophilus influenzae and other bacillary meningitides was attempted by gas-liquid chromatography (GLC) of the metabolic by-products in broth cultures and in cerebrospinal fluid (CSF) samples obtained from experimental meningitis produced in New Zealand White male rabbits. These results were correlated with the GLC of CSF of meningitis patients. A major peak with retent...

Fermentation Media by Gas-Liquid Chromatography

PACKETT, L. V. (Purdue University, Lafayette, Ind.), AND R. W. MCCUNE. Determination of steam-volatile organic acids in fermentation media by gas-liquid chromatography. Appl. Microbiol. 13:22-27. 1965.-Five gas chromatographic liquid phases (25% Carbowax 20M plus 4% H3PO4, 17.5% dioctyl sebacate plus 7.5% sebacic acid, 17.5% dioctyl sebacate plus 7.5% docosanoic acid, 5% Tween 80, and 20% LAC29...

Hippurate Hydrolysis by Gas-Liquid Chromatography

A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37°C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive,...

Gas and Liquid Chromatography